Substrate specificity of the rat liver Na1-bile salt cotransporter in Xenopus laevis oocytes and in CHO cells
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چکیده
ALICE SCHROEDER,1 UTA ECKHARDT,1 BRUNO STIEGER,1 RONALD TYNES,2 CLAUDIO D. SCHTEINGART,3,4 ALAN F. HOFMANN,3 PETER J. MEIER,1 AND BRUNO HAGENBUCH1 1Division of Clinical Pharmacology and Toxicology, Department of Medicine, University Hospital, CH-8091 Zurich; 2Drug Metabolism and Pharmakokinetics, Novartis Pharma, CH-4002 Basel, Switzerland; 3Division of Gastroenterology, Department of Medicine, University of California San Diego, La Jolla 92093-0813; and 4Ferring Research, La Jolla, California 92037
منابع مشابه
Substrate specificity of the rat liver Na+-bile salt cotransporter in Xenopus laevis oocytes and in CHO cells.
It has been proposed that the hepatocellular Na+-dependent bile salt uptake system exhibits a broad substrate specificity in intact hepatocytes. In contrast, recent expression studies in mammalian cell lines have suggested that the cloned rat liver Na+-taurocholate cotransporting polypeptide (Ntcp) may transport only taurocholate. To characterize its substrate specificity Ntcp was stably transf...
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Nagata, Katsumi, Naohiro Hori, Kenzo Sato, Kunimasa Ohta, Hideaki Tanaka, and Yasutake Hiji. Cloning and functional expression of an SGLT-1-like protein from the Xenopus laevis intestine. Am. J. Physiol. 276 (Gastrointest. Liver Physiol. 39): G1251–G1259, 1999.—A cDNA encoding an Na1-glucose cotransporter type 1 (SGLT-1)-like protein was cloned from the Xenopus laevis intestine by the 58and 38-...
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The expression of the basolateral Na+/bile acid (taurocholate) cotransport system of rat hepatocytes has been studied in Xenopus laevis oocytes. Injection of rat liver poly(A)+ RNA into the oocytes resulted in the functional expression of Na+ gradient stimulated taurocholate uptake within 3-5 days. This Na(+)-dependent portion of taurocholate uptake exhibited saturation kinetics (apparent Km ap...
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A Na(+)-dependent bile acid (Na+/taurocholate co-transporting polypeptide; Ntcp) and a Na(+)-independent bromosulphophthalein (BSP)/bile acid uptake system (organic-anion-transporting polypeptide; oatp) have been cloned from rat liver by using functional expression cloning in Xenopus laevis oocytes. To evaluate the extent to which these cloned transporters could account for overall hepatic bile...
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Abstract The S362A mutation block ROMK2 (Kir1.1b) endocytosis in Xenopus laevis oocyte membrane . Saeed Hajihashemi1 , 1-Assistant professor, PhD in Physiology, Department of Physiology, School of Medical science, Arak University of Medical Sciences. Introduction: ROMK channel is localized on the apical membrane of the nephron. Recent studies suggest that endocytosis of ROMK chan...
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